Isolation of Homogenous Population of Human Amniotic Epithelial Stems Cells
نویسندگان
چکیده
منابع مشابه
isolation and partial characterization of human amniotic epithelial cells: the effect of trypsin
background: despite the extensive information available in the literature, cell surface marker signature of human amniotic epithelial cells (haecs) remains controversial. the aim of the present study was to characterize immuno-phenotypic features, proliferative capacity and immunogenicity of haecs. we also tested whether expression of some cell surface markers is influenced by the type of tryps...
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The human amniotic membrane known as the innermost single epithelial-covered layer provides many applications such as applicable anti-inflammatory and anti-cancer effects. These immunomodulatory effects belongs to the epithelial cells, a type of epiblast-derived fetal stem cells which currently used for regenerative medicine and transplantation. These cells are collected by author-prepared faci...
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Hertwig's epithelial root sheath (HERS) cells play a pivotal role during root formation of the tooth and are able to form cementum-like tissue. The aim of the present study was to establish a HERS cell line for molecular and biochemical studies using a selective digestion method. Selective digestion was performed by the application of trypsin-EDTA for 2 min, which led to the detachment of fibro...
متن کاملConstruction of tissue engineered skin with human amniotic mesenchymal stem cells and human amniotic epithelial cells.
OBJECTIVE To establish a new model for construction of tissue engineered skin with human amniotic mesenchymal stem cells (hAMSCs) and human amniotic epithelial cells (hAECs). MATERIALS AND METHODS hAMSCs and hAECs were isolated from amniotic membrane. The morphology and phenotype of hAMSCs and hAECs were confirmed by microscope and flow cytometry, respectively. Then, we performed RT-PCR and i...
متن کاملIsolation and Partial Characterization of Human Amniotic Epithelial Cells: The Effect of Trypsin
BACKGROUND Despite the extensive information available in the literature, cell surface marker signature of human Amniotic Epithelial Cells (hAECs) remains controversial. The aim of the present study was to characterize immunophenotypic features, proliferative capacity and immunogenicity of hAECs. We also tested whether expression of some cell surface markers is influenced by the type of trypsin...
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ژورنال
عنوان ژورنال: Pakistan journal of medicine and dentistry
سال: 2022
ISSN: ['2308-2593', '2313-7371']
DOI: https://doi.org/10.36283/pjmd11-3/009